Asparaginase (Escherichia coli)
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MANUFACTURER: MEGAZYME
PRODUCT CODE: E-ASNEC
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Description
Asparaginase (Escherichia coli)
Highlight for Asparaginase (Escherichia coli)
| Content: | 400 Units at 25oC |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 2.5 M lithium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Other Activities |
| EC Number: | 3.5.1.1 |
| CAS Number: | 9015-68-3 |
| Source: | Escherichia coli |
| Molecular Weight: | 37,900 |
| Concentration: | Supplied at ~ 350 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Catalyses the reaction: L-Asparagine + H2O = L-aspartate + NH3 |
| Specific Activity: | ~ 15 U/mg (25oC, pH 8.0 on L-asparagine); ~ 30 U/mg (37oC, pH 8.0 on L-asparagine) |
| Unit Definition: | One Unit of asparaginase activity is defined as the amount of enzyme required to produce one µmole of L-aspartate from L-asparagine (7.3 mM) per minute in the presence of NADPH in Tris.HCL buffer (45 mM), pH 8.0. |
